自体荧光
生物
荧光
荧光寿命成像显微镜
分子成像
共焦显微镜
植物细胞
显微镜
基因表达
计算生物学
生物物理学
基因
细胞生物学
生物化学
生物技术
光学
物理
体内
作者
Daisuke Kurihara,Yoko Mizuta,Yoshikatsu Sato,Tetsuya Higashiyama
出处
期刊:Development
[The Company of Biologists]
日期:2015-01-01
被引量:403
摘要
Imaging techniques for visualizing and analyzing precise morphology and gene expression patterns are essential for understanding biological processes during development in all organisms. With the aid of chemical screening, we developed a clearing method using chemical solutions, termed ClearSee, for deep imaging of morphology and gene expression in plant tissues. ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting the refractive index mismatch, whole-organ and whole-plant imaging can be performed by both confocal and two-photon excitation microscopy in ClearSee-treated samples. Moreover, ClearSee is applicable to multicolor imaging of fluorescent proteins to allow structural analysis of multiple gene expression. Given that ClearSee is compatible with staining by chemical dyes, the technique is useful for deep imaging in conjunction with genetic markers and for plant species not amenable to transgenic approaches. This method is useful for whole imaging for intact morphology and will help to accelerate the discovery of new phenomena in plant biological research.
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