The Arabidopsis thaliana gametophytic mutation gemini pollen1 disrupts microspore polarity, division asymmetry and pollen cell fate

生物 小孢子 拟南芥 花粉 细胞分裂 极性(国际关系) 细胞命运测定 拟南芥 配子体 细胞生物学 植物 突变 遗传学 细胞 雄蕊 基因 突变体 转录因子
作者
Soon Ki Park,Ross Howden,David Twell
出处
期刊:Development [The Company of Biologists]
卷期号:125 (19): 3789-3799 被引量:245
标识
DOI:10.1242/dev.125.19.3789
摘要

Pollen development and male gametogenesis are critically dependent upon cell polarization leading to a highly asymmetric cell division termed pollen mitosis I. A mutational approach was adopted in Arabidopsis thaliana to identify genes involved these processes. Four independent gemini pollen mutants were isolated which produce divided or twin-celled pollen. The gemini pollen1 mutant was characterized in detail and shown to act gametophytically resulting in reduced transmission through both sexes. gemini pollen1 showed an incompletely penetrant phenotype resulting in equal, unequal and partial divisions at pollen mitosis I. The division planes in gemini pollen1 were shown to be aligned with the polar axis (as in wild type) and evidence was obtained for incomplete nuclear migration, which could account for altered division symmetry. gemini pollen1 also showed division phenotypes consistent with spatial uncoupling of karyokinesis and cytokinesis suggesting that GEMINI POLLEN1 may be required for the localization of phragmoplast activity. Cell fate studies showed that in both equal and unequal divisions a vegetative cell marker gene was activated in both daughter cells. Daughter cells with a range of intermediate or hybrid vegetative/generative cell fates suggests that cell fate is quantitatively related to cell size. The potential mode of action of GEMINI POLLEN1 and its effects on cell fate are discussed in relation to proposed models of microspore polarity and cell fate determination.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
鲜于飞薇发布了新的文献求助10
1秒前
FashionBoy应助刘佳敏采纳,获得10
2秒前
SYLH应助gjw采纳,获得10
3秒前
风轻萤发布了新的文献求助10
3秒前
LXL0125完成签到,获得积分10
3秒前
传奇3应助自觉紫安采纳,获得10
4秒前
4秒前
尤幻雪发布了新的文献求助30
4秒前
朴实的悲完成签到,获得积分20
4秒前
笑点低的小天鹅完成签到,获得积分10
4秒前
老实鞯完成签到 ,获得积分20
5秒前
5秒前
略略略发布了新的文献求助10
5秒前
赘婿应助momo采纳,获得10
5秒前
青衣北风发布了新的文献求助10
6秒前
wdlc发布了新的文献求助20
6秒前
hhj发布了新的文献求助10
6秒前
6秒前
7秒前
弗洛洛发布了新的文献求助20
7秒前
深情安青应助饱满的靖易采纳,获得30
8秒前
英姑应助molamola采纳,获得10
8秒前
bkagyin应助万万没想到采纳,获得10
8秒前
xiaotudou95发布了新的文献求助10
9秒前
zlzl完成签到,获得积分10
10秒前
怕孤单的问雁完成签到,获得积分10
10秒前
Druid发布了新的文献求助10
10秒前
gjw完成签到,获得积分10
10秒前
11秒前
曦宝儿完成签到,获得积分10
11秒前
华仔应助AZE采纳,获得20
12秒前
廖彬彬完成签到,获得积分20
12秒前
风轻萤完成签到,获得积分10
12秒前
JamesPei应助漂流的云朵采纳,获得10
12秒前
悦悦完成签到,获得积分10
12秒前
社恐小青年完成签到,获得积分20
13秒前
13秒前
123发布了新的文献求助10
13秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971091
求助须知:如何正确求助?哪些是违规求助? 3515797
关于积分的说明 11179488
捐赠科研通 3250872
什么是DOI,文献DOI怎么找? 1795536
邀请新用户注册赠送积分活动 875891
科研通“疑难数据库(出版商)”最低求助积分说明 805207