Chloride Ions Stabilize Human Adult Hemoglobin in the T-State, Competing with Allosteric Interaction of Oxygen Molecules

变构调节 化学 背景(考古学) 氯化物 分子 血红蛋白 离子 氧气 生物物理学 立体化学 生物化学 有机化学 生物 古生物学
作者
Ikuo Kurisaki,Yume Takahashi,Yuichi Kitamura,Masataka Nagaoka
出处
期刊:Journal of Physical Chemistry B [American Chemical Society]
卷期号:125 (46): 12670-12677 被引量:1
标识
DOI:10.1021/acs.jpcb.1c07520
摘要

In the context of a molecular-level understanding of the allostery mechanisms, human adult hemoglobin (HbA) has been extensively studied for over half a century. Chloride ions (Cl–) have been known as one of HbA allosteric effectors, which stabilizes the T-state preferable to release oxygen molecules. The functional mechanisms were individually proposed by Ueno and Perutz several decades ago. Ueno considered that the site-specific Cl– binding is essential, while Perutz proposed the non-site-specific interaction between HbA and Cl–. Each speculation explains the mechanism plausibly since each was tightly associated with its reasonable experimental observation. However, both mechanisms themselves still seem to make their speculations controversial. In the present study, we have theoretically reconsidered these apart from their approaches. Our atomistic molecular dynamics simulations then showed that the increase of Cl– concentration suppresses the conformational conversion from the T-state. Interestingly, chloride ions loosely interact with the amino acid residues inside the HbA central cavity, suggesting that both Perutz's and Ueno's speculations are involved in understanding the microscopic roles of Cl–. In conclusion, we theoretically certified that the effect of Cl– competes against that of solvated O2, i.e., the destabilization of T-state through the non-site-specific interaction, implying the concerted regulation of HbA under physiological conditions.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
传奇3应助明朗采纳,获得10
1秒前
1秒前
1秒前
2秒前
senhoo发布了新的文献求助10
2秒前
2秒前
小狗完成签到 ,获得积分10
3秒前
4秒前
4秒前
昏睡的实验室小白完成签到,获得积分10
5秒前
5秒前
yar应助大青山采纳,获得10
5秒前
Rondab应助mariawang采纳,获得10
5秒前
yqb发布了新的文献求助10
5秒前
5秒前
一条裸游的鱼完成签到,获得积分10
7秒前
二白关注了科研通微信公众号
7秒前
8秒前
8秒前
超帅听枫发布了新的文献求助10
8秒前
10秒前
10秒前
fish1116发布了新的文献求助100
10秒前
10秒前
bbbbb沫完成签到,获得积分20
10秒前
峥2发布了新的文献求助10
10秒前
wzy完成签到,获得积分10
10秒前
xxxllllll发布了新的文献求助10
11秒前
干净映天完成签到 ,获得积分10
11秒前
LJ_scholar发布了新的文献求助10
12秒前
kuny完成签到,获得积分10
12秒前
勤恳的夏之完成签到,获得积分20
13秒前
wzy发布了新的文献求助10
13秒前
钵钵鸡完成签到 ,获得积分10
13秒前
JING发布了新的文献求助10
13秒前
後zgw完成签到,获得积分10
15秒前
15秒前
16秒前
明朗发布了新的文献求助10
16秒前
meredith0571完成签到,获得积分10
17秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988732
求助须知:如何正确求助?哪些是违规求助? 3531027
关于积分的说明 11252281
捐赠科研通 3269732
什么是DOI,文献DOI怎么找? 1804764
邀请新用户注册赠送积分活动 881869
科研通“疑难数据库(出版商)”最低求助积分说明 809021