粘细菌
黄色粘球菌
计算生物学
生物
发起人
基因
基因组
合成生物学
遗传学
作者
Weifeng Hu,Luo Niu,Yue X,Le-Le Zhu,Wei Hu,Yue‐zhong Li,Changsheng Wu
标识
DOI:10.1021/acssynbio.1c00444
摘要
Genome mining has revealed that myxobacteria contain a myriad of cryptic biosynthetic gene clusters (BGCs). Here, we report the characterization of a panel of myxobacterial promoters with variable strength that are applicable in the engineering of BGCs in myxobacteria. The screened strongest constitutive promoter was used to efficiently enhance the expression of two complex BGCs governing the biosynthesis of myxochromide and DKxanthene in the model myxobacterium Myxococcus xanthus DK1622. We also showcased the combination of promoter engineering and MS2-based spectral networking as an effective strategy to shed light on the previously overlooked chemistry in the family of myxochromide-type lipopeptides. The enriched promoter library substantially expanded the synthetic biology toolkit available for myxobacteria.
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