Ultrasensitive and practical chemiluminescence sensing pesticide residue acetamiprid in agricultural products and environment: Combination of synergistically coupled co-amplifying signal and smart interface engineering

Practical detection of single-component pesticide residue at ultra-low concentrations in agricultural products and environment is very important for assessment of environmental risks and protection of human health. Herein, a practical and highly sensitive chemiluminescence (CL) sensing acetamiprid in agricultural products and environmental media was constructed based on the synergistic co-catalysis of graphene oxide (GO)/gold nanoparticles (AuNPs) nanocomposites for luminol CL reaction and the smart interface engineering. ss-DNA could inhibit co-catalysis of GO/AuNPs for luminol CL reaction. Once acetamiprid was added, aptamer conformation changed in dimension and synergistically catalytic amplification signal of GO/AuNPs was restored significantly. The limit of detection was 8.9 pM. High sensitivity could be due to strong signal amplification from synergistic catalysis of GO/AuNPs for CL reaction and perfect regulation of composite interface by DNA dimension. Moreover, the used GO/AuNPs could be stably stored for six months, which was superior to previously reported AuNPs (only half a month). The analysis exhibited excellent selectivity for acetamiprid. The detection results for real samples confirmed reliability in practical application. This analysis is an extremely useful method for monitoring pesticide residues in environment and agricultural products. Synergetic co-catalysis of GO/AuNPs and ingenious interface engineering provide important ideas for other biosensors.