神经血管束
干细胞
神经科学
冲程(发动机)
医学
小胶质细胞
生物医学工程
生物
细胞生物学
病理
炎症
内科学
机械工程
工程类
作者
Zhonglin Lyu,Jon Park,Kwangmin Kim,Hye-jin Jin,Haodi Wu,Jayakumar Rajadas,Deok‐Ho Kim,Gary K. Steinberg,Wonjae Lee
标识
DOI:10.1038/s41551-021-00744-7
摘要
The therapeutic efficacy of stem cells transplanted into an ischaemic brain depends primarily on the responses of the neurovascular unit. Here, we report the development and applicability of a functional neurovascular unit on a microfluidic chip as a microphysiological model of ischaemic stroke that recapitulates the function of the blood–brain barrier as well as interactions between therapeutic stem cells and host cells (human brain microvascular endothelial cells, pericytes, astrocytes, microglia and neurons). We used the model to track the infiltration of a number of candidate stem cells and to characterize the expression levels of genes associated with post-stroke pathologies. We observed that each type of stem cell showed unique neurorestorative effects, primarily by supporting endogenous recovery rather than through direct cell replacement, and that the recovery of synaptic activities is correlated with the recovery of the structural and functional integrity of the neurovascular unit rather than with the regeneration of neurons. A microphysiological model of ischaemic stroke consisting of a functional neurovascular unit on a microfluidic chip allows for the systematic characterization of the neurorestorative outcomes of candidate stem cell therapies.
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