生物正交化学
菁
细胞内
化学
费斯特共振能量转移
扫描电镜
生物物理学
荧光
部分
能量转移
光化学
纳米技术
组合化学
点击化学
生物化学
材料科学
立体化学
受激发射
化学物理
激光器
物理
光学
量子力学
生物
作者
Evelin Albitz,Dóra Kern,Attila Kormos,Márton Bojtár,György Török,Adrienn Bíró,Ágnes Szatmári,Krisztina Németh,Péter Kele
标识
DOI:10.1002/anie.202111855
摘要
An energy transfer-based signal amplification relay concept enabling transmission of bioorthogonally activatable fluorogenicity of blue-excitable coumarins to yellow/red emitting cyanine frames is presented. Such relay mechanism resulted in improved cyanine fluorogenicities together with increased photostabilities and large apparent Stokes-shifts allowing lower background fluorescence even in no-wash bioorthogonal fluorogenic labeling schemes of intracellular structures in live cells. These energy transfer dyads sharing the same donor moiety together with their parent donor molecule allowed three-color imaging of intracellular targets using one single excitation source with separate emission windows. Sub-diffraction imaging of intracellular structures using the bioorthogonally activatable FRET dyads by STED microscopy is also presented.
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