Melatonin promotes potassium deficiency tolerance by regulating HAK1 transporter and its upstream transcription factor NAC71 in wheat

基因沉默 转录因子 生物 细胞生物学 下调和上调 基因表达 基因 分子生物学 遗传学
作者
Gezi Li,Jin Liu,Shi‐Juan Chen,Pengfei Wang,Hai‐Tao Liu,Jie Dong,Yong‐Xing Zheng,Yingxin Xie,Chenyang Wang,Tiancai Guo,Guozhang Kang
出处
期刊:Journal of Pineal Research [Wiley]
卷期号:70 (4) 被引量:24
标识
DOI:10.1111/jpi.12727
摘要

Abstract Melatonin (MT) is involved in various physiological processes and stress responses in animals and plants. However, little is known about the molecular mechanisms by which MT regulates potassium deficiency (DK) tolerance in crops. In this study, an appropriate concentration (50 μmol/L) was found to enhance the tolerance of wheat plants against DK. RNA‐seq analysis showed that a total of 6253 and 5873 differentially expressed genes (DEGs) were separately identified in root and leaf tissues of the DK + MT‐treated wheat plants. They functionally involved biological processes of secondary metabolite, signal transduction, and transport or catabolism. Of these, an upregulated high‐affinity K transporter 1 ( TaHAK1 ) gene was next characterized. TaHAK1 overexpression markedly enhanced the K absorption, while its transient silencing exhibited the opposite effect, suggesting its important role in MT‐mediated DK tolerance. Moreover, yeast one‐hybrid (Y1H) was used to screen the upstream regulators of TaHAK1 gene and the transcription factor TaNAC71 was identified. The binding between TaNAC71 and TaHAK1 promoter was evidenced by using Y1H, LUC, and EMSA assays. Transient overexpression of TaNAC71 in wheat protoplasts activated the TaHAK1 expression, whereas its transient silencing inhibited the TaHAK1 expression and aggravated the sensitivity to DK. Exogenous MT application greatly upregulated the expression of TaHAK1 in both transient overexpression and silencing systems. Our findings revealed some molecular mechanisms underlying MT‐mediated DK tolerance and helped broaden its practical application in agriculture.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小黄发布了新的文献求助10
刚刚
JamesPei应助无奈聪展采纳,获得10
1秒前
田様应助July采纳,获得10
1秒前
乐乐应助July采纳,获得10
1秒前
平仄完成签到,获得积分10
1秒前
2秒前
雨天完成签到 ,获得积分10
2秒前
忧虑的盼望发布了新的文献求助200
3秒前
yingjin发布了新的文献求助10
8秒前
陈陈完成签到,获得积分10
12秒前
hui发布了新的文献求助30
16秒前
yin应助July采纳,获得10
16秒前
yin应助July采纳,获得10
17秒前
情怀应助July采纳,获得10
17秒前
heavenhorse应助July采纳,获得30
17秒前
是昭昭呀应助July采纳,获得10
17秒前
SYLH应助July采纳,获得10
17秒前
弄啥嘞昂应助July采纳,获得10
17秒前
不安青牛应助July采纳,获得10
17秒前
香蕉觅云应助July采纳,获得10
17秒前
香蕉觅云应助July采纳,获得10
17秒前
一只小胖橘完成签到 ,获得积分10
17秒前
19秒前
fun发布了新的文献求助10
19秒前
暖暖圆圆发布了新的文献求助10
20秒前
丘比特应助高高的觅风采纳,获得10
21秒前
21秒前
所所应助瞿选葵采纳,获得10
21秒前
平仄关注了科研通微信公众号
24秒前
发一篇sci发布了新的文献求助10
26秒前
陶醉觅夏发布了新的文献求助10
26秒前
英姑应助早晚炸了学校采纳,获得10
26秒前
FIN应助HYD采纳,获得10
26秒前
慕青应助幽默的宛白采纳,获得10
26秒前
小孙发布了新的文献求助10
27秒前
28秒前
lierking应助July采纳,获得10
30秒前
劲秉应助July采纳,获得10
30秒前
Accept应助July采纳,获得10
30秒前
大模型应助July采纳,获得10
30秒前
高分求助中
Востребованный временем 2500
Agaricales of New Zealand 1: Pluteaceae - Entolomataceae 1040
지식생태학: 생태학, 죽은 지식을 깨우다 600
海南省蛇咬伤流行病学特征与预后影响因素分析 500
Neuromuscular and Electrodiagnostic Medicine Board Review 500
ランス多機能化技術による溶鋼脱ガス処理の高効率化の研究 500
Relativism, Conceptual Schemes, and Categorical Frameworks 500
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3462603
求助须知:如何正确求助?哪些是违规求助? 3056160
关于积分的说明 9050826
捐赠科研通 2745793
什么是DOI,文献DOI怎么找? 1506578
科研通“疑难数据库(出版商)”最低求助积分说明 696165
邀请新用户注册赠送积分活动 695677