化学
分析化学(期刊)
胶束
临界胶束浓度
鼹鼠
核化学
钠
色谱法
物理化学
有机化学
水溶液
作者
Sunisa Kaewpaiboon,Teerapol Srichana
标识
DOI:10.1016/j.xphs.2022.02.011
摘要
Polymyxin B (PMB) and sodium deoxycholate sulfate (SDCS) ratios were optimized. The self-assembly of PMB-SDCS was characterized using dynamic light scattering. Five different mole ratios of SDCS to PMB (5:1, 10:1, 15:1, 30:1, and 45:1) were prepared after optimization. FTIR and 1H-NMR were employed to characterize PMB formulations. The chemical stability of PMB was quantified with tandem mass spectrometry. Both PMB and SDCS formed micelles at 14 and 8 μg/ml, respectively. At the critical micelle concentration (CMC), the hydrodynamic diameter of 213 nm was obtained. PMB had a positive charge (+6 mV) while SDCS had a negative charge (‒33 mV). Increasing in SDCS content decreased the charges from ‒6 to ‒25 mV. FTIR revealed H-bonding between PMB and SDCS. The NMR spectra confirmed that chemical shifts of PMB and SDCS did not change. The hydrodynamic size of PMB-SDCS was from 193 to 318 d.nm. Our results suggest that the lower mole ratios of SDCS (< 15:1) were able to stabilize PMB and released PMB within 30 min. Moreover, 5:1 mole ratio maintained the antimicrobial activity against Pseudomonas aeruginosa (MBC = 2 μg/ml). PMB-SDCS micelles of particular mole ratio is able to provide physical and chemical stability of PMB.
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