New Developments in Quantitative Real-time Polymerase Chain Reaction Technology

A summary of the developments of the last few years in RT-qPCR technology and nucleic acid amplification. RT-qPCR technology has revolutionized the detection landscape in every area of molecular biology. Various modifications have enhanced the overall performance. These range from changes in the individual components of the enzymatic reaction cocktail (polymerizing enzymes, reaction buffers, probes, etc.) to the detection system itself (instrumentation, software, etc.). RT-qPCR technology is now more sensitive, faster and affordable.