新月形茎杆菌
突变
基因组
生物
遗传学
全基因组测序
突变
插入突变
基因
计算生物学
细菌蛋白
作者
Ingrid Reale Alves,Ricardo Z. N. Vêncio,Rodrigo S. Galhardo
标识
DOI:10.1016/j.mrfmmm.2022.111787
摘要
UV-induced mutagenesis is, to greater extent, a phenomenon dependent on translesion synthesis (TLS) and regulated by the SOS response in bacteria. Caulobacter crescentus, like many bacterial species, employs the ImuABC (ImuAB DnaE2) pathway in TLS. To have a better understanding of the characteristics of UV-induced mutagenesis in this organism, we performed a whole genome analysis of mutations present in survivors after an acute UVC exposure (300 J/m2). We found an average of 3.2 mutations/genome in irradiated samples, distributed in a mutational spectrum consisting exclusively of base substitutions, including tandem mutations. Although limited in conclusions by the small number of mutations identified, our study points to the feasibility of using whole-genome sequencing to study mutagenesis occurring in experiments involving a single acute exposure to genotoxic agents.
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