色域
异染色质蛋白1
染色质
组蛋白H3
EZH2型
组蛋白甲基化
组蛋白甲基转移酶
表观遗传学
细胞生物学
组蛋白
组蛋白密码
组蛋白H2A
生物
化学
分子生物学
DNA甲基化
遗传学
核小体
异染色质
基因表达
基因
解旋酶
核糖核酸
作者
Kazuki Sasaki,Michihiro Suzuki,Takeshi Sonoda,Tilman Schneider‐Poetsch,Akihiro Ito,Motoki Takagi,Shinya Fujishiro,Yoshihiro Sohtome,Kosuke Dodo,Takashi Umehara,Hiroyuki Aburatani,Kazuo Shin‐ya,Yoichi Nakao,Mikiko Sodeoka,Minoru Yoshida
标识
DOI:10.1016/j.chembiol.2022.05.006
摘要
Histone lysine methylation is an epigenetic mark that can control gene expression. In particular, H3K9me3 contributes to transcriptional repression by regulating chromatin structure. Successful mitotic progression requires correct timing of chromatin structure changes, including epigenetic marks. However, spatiotemporal information on histone modifications in living cells remains limited. In this study, we created an FRET-based probe for live-cell imaging based on the HP1α chromodomain (HP1αCD), which binds to H3K9me3. The probe was incorporated into chromatin and the emission ratio decreased after treatment with histone methyltransferase inhibitors, indicating that it successfully traced dynamic changes in H3K9me3. Upon entry into mitosis, the probe's emission ratio transiently increased with a concomitant increase in H3K9me3, then exhibited a stepwise decrease, probably due to loss of HP1αCD binding caused by phosphorylation of H3S10 and demethylation of H3K9me3. This probe will be a useful tool for detecting dynamic changes in chromatin structure associated with HP1α.
科研通智能强力驱动
Strongly Powered by AbleSci AI