二化螟
阿维菌素
ATP结合盒运输机
生物
戒毒(替代医学)
运输机
九氟化硫
生物化学
生物测定
杀虫剂
基因
植物
遗传学
幼虫
生态学
夜蛾
替代医学
病理
医学
重组DNA
作者
Daojie Guan,Xuemei Yang,Heng Jiang,Nan Zhang,Zhaolu Wu,Chengyun Jiang,Qinwen Shen,Kun Qian,Jianjun Wang,Xiangkun Meng
标识
DOI:10.1021/acs.jafc.2c00414
摘要
Chilo suppressalis has developed high levels of resistance to abamectin in many areas of China, while the underline resistance mechanisms are largely unclear. ATP-binding cassette (ABC) transporters function in transporting a large diversity of substrates including insecticides and play important roles in the detoxification metabolism of insects. In this study, synergism bioassay revealed that the ABC transporters were involved in the detoxification of C. suppressalis to abamectin. Six ABC transporter genes were upregulated in C. suppressalis after abamectin exposure, among which five genes CsABCC8, CsABCE1, CsABCF1, CsABCF2, and CsABCH1 were induced in the detoxification-related tissues. In addition, the five ABC transporters were recombinantly expressed in Sf9 cells, and the cytotoxicity assay showed that the viabilities of cells expressing CsABCC8 or CsABCH1 were significantly increased when compared with the viabilities of cells expressing EGFP after abamectin, chlorantraniliprole, cyantraniliprole, fipronil, and chlorpyrifos treatment, respectively. Overexpression of CsABCE1 significantly increased the viabilities of cells to abamectin, chlorantraniliprole, deltamethrin, and indoxacarb exposure, respectively. These results suggested that CsABCC8, CsABCE1, and CsABCH1 might participate in the detoxification and transport of abamectin and several other classes of insecticides in C. suppressalis. Our study provides valuable insights into the transport-related detoxification mechanisms in C. suppressalis and other insects.
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