23S核糖体RNA
基因分型
肺炎支原体
生物
基因
支原体
微生物学
基因突变
点突变
遗传学
基因型
突变
医学
核糖核酸
核糖体
内科学
肺炎
作者
Yu Suzuki,Junji Seto,Yoshitaka Shimotai,Tatsuya Ikeda,Kazue Yahagi,Katsumi Mizuta,Yoko Matsuzaki,Seiji Hongo
标识
DOI:10.1016/j.mimet.2016.10.017
摘要
The prevalence of macrolide-resistant Mycoplasma pneumoniae harboring a mutation in the 23S rRNA gene is increasing, and rapid detection assays are needed for clinical management. We developed an endpoint genotyping assay to detect the M. pneumoniae 23S rRNA gene and determine the existence of macrolide resistance-associated mutations at position 2063 (A2063G, A2063T and A2063C mutations). This A2063B genotyping assay detected more than 50 copies/reaction of the M. pneumoniae gene in every nucleotide mutation at position 2063. Of 42 clinical specimens, 3 were positive without mutation, 6 were positive with the A2063G mutation, and 33 were negative. The results were confirmed using nested PCR with the sequencing of the M. pneumoniae 23S rRNA gene, and a high sensitivity (90%), specificity (100%), and coincidence ratio (kappa coefficient=0.93) were obtained. Therefore, the A2063B genotyping assay is useful for the rapid discrimination of macrolide resistance mutations at position 2063.
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