Glycoproteomic markers of hepatocellular carcinoma‐mass spectrometry based approaches

肝细胞癌 岩藻糖基化 肝硬化 化学 血红素 肝癌 聚糖 癌症 酒精性肝炎 内科学 酒精性肝病 医学 糖蛋白 生物化学 血红素
作者
Jianhui Zhu,Elisa Warner,Neehar D. Parikh,David M. Lubman
出处
期刊:Mass Spectrometry Reviews [Wiley]
卷期号:38 (3): 265-290 被引量:67
标识
DOI:10.1002/mas.21583
摘要

Hepatocellular carcinoma (HCC) is the third most‐common cause of cancer‐related death worldwide. Most cases of HCC develop in patients that already have liver cirrhosis and have been recommended for surveillance for an early onset of HCC. Cirrhosis is the final common pathway for several etiologies of liver disease, including hepatitis B and C, alcohol, and increasingly non‐alcoholic fatty liver disease. Only 20–30% of patients with HCC are eligible for curative therapy due primarily to inadequate early‐detection strategies. Reliable, accurate biomarkers for HCC early detection provide the highest likelihood of curative therapy and survival; however, current early‐detection methods that use abdominal ultrasound and serum alpha fetoprotein are inadequate due to poor adherence and limited sensitivity and specificity. There is an urgent need for convenient and highly accurate validated biomarkers for HCC early detection. The theme of this review is the development of new methods to discover glycoprotein‐based markers for detection of HCC with mass spectrometry approaches. We outline the non‐mass spectrometry based methods that have been used to discover HCC markers including immunoassays, capillary electrophoresis, 2‐D gel electrophoresis, and lectin‐FLISA assays. We describe the development and results of mass spectrometry‐based assays for glycan screening based on either MALDI‐MS or ESI analysis. These analyses might be based on the glycan content of serum or on glycan screening for target molecules from serum. We describe some of the specific markers that have been developed as a result, including for proteins such as Haptoglobin, Hemopexin, Kininogen, and others. We discuss the potential role for other technologies, including PGC chromatography and ion mobility, to separate isoforms of glycan markers. Analyses of glycopeptides based on new technologies and innovative softwares are described and also their potential role in discovery of markers of HCC. These technologies include new fragmentation methods such as EThcD and stepped HCD, which can identify large numbers of glycopeptide structures from serum. The key role of lectin extraction in various assays for intact glycopeptides or their truncated versions is also described, where various core‐fucosylated and hyperfucosylated glycopeptides have been identified as potential markers of HCC. Finally, we describe the role of LC‐MRMs or lectin‐FLISA MRMs as a means to validate these glycoprotein markers from patient samples. These technological advancements in mass spectrometry have the potential to lead to novel biomarkers to improve the early detection of HCC.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
月秋发布了新的文献求助10
2秒前
复杂的夜蓉完成签到,获得积分10
2秒前
yoona完成签到,获得积分10
3秒前
3秒前
qks发布了新的文献求助10
4秒前
泡芙不甜完成签到 ,获得积分10
4秒前
5秒前
7秒前
7秒前
冬瓜完成签到 ,获得积分10
8秒前
Fu发布了新的文献求助10
8秒前
tuanheqi应助旷野天采纳,获得30
9秒前
9秒前
材料诚完成签到,获得积分10
9秒前
LabRat完成签到 ,获得积分10
9秒前
caibai发布了新的文献求助10
10秒前
幽默不乐完成签到,获得积分20
10秒前
Bzz完成签到,获得积分10
11秒前
12秒前
研友_VZG7GZ应助乐白采纳,获得10
12秒前
oldfive发布了新的文献求助10
12秒前
13秒前
在水一方应助月秋采纳,获得10
13秒前
小鸟芄完成签到,获得积分10
13秒前
Michael发布了新的文献求助10
14秒前
阔达的扬完成签到,获得积分10
14秒前
琪琪的发布了新的文献求助10
15秒前
HanQing完成签到,获得积分20
15秒前
17秒前
18秒前
丘比特应助不过尔尔采纳,获得10
19秒前
自觉秋发布了新的文献求助10
19秒前
小贺完成签到,获得积分10
21秒前
学术小白发布了新的文献求助10
21秒前
N7完成签到,获得积分10
21秒前
qgjvjypm发布了新的文献求助10
21秒前
22秒前
搜集达人应助枯木逢春采纳,获得10
22秒前
Michael完成签到,获得积分20
23秒前
四川知名猛男完成签到 ,获得积分10
23秒前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3101389
求助须知:如何正确求助?哪些是违规求助? 2752795
关于积分的说明 7621022
捐赠科研通 2405111
什么是DOI,文献DOI怎么找? 1276127
科研通“疑难数据库(出版商)”最低求助积分说明 616705
版权声明 599058