化学
荧光
抗坏血酸
一氧化氮
共轭体系
生物物理学
内生
半胱氨酸
光化学
电子转移
细胞内
组合化学
聚合物
生物化学
有机化学
酶
量子力学
生物
物理
食品科学
作者
Qi Zhao,Hao Zhao,Yang Guo,Ziqi Zhang,You Hu,Yanli Tang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2018-10-16
卷期号:90 (21): 12663-12669
被引量:16
标识
DOI:10.1021/acs.analchem.8b02891
摘要
Nitric oxide (NO) is a celebrated signaling molecule involved in diverse vital physiological and pathophysiological processes. Thus, rapid detection of NO in situ is quite significant due to its large diffusion and short half-time. Herein, a new water-soluble conjugated polymer (PBFB-PDA-NMe3+) with an o-diaminophenyl group in the side chain is designed and synthesized as a fluorescence probe for ultrarapid and sensitive detection of endogenous NO via photoinduced electron transfer (PET) mechanism. In the presence of NO, NO can be specifically trapped by o-diaminophenyl group and react with it to form benzotriazole derivative, which leads to the PET being inhibited. The fluorescence of probe thus significantly turns on. Most importantly, the assay is completed within 1 min without further treatments. Furthermore, this probe can rapidly image intracellular NO in A549 cell and S. aureus bacteria only incubating for 15 min. In addition, this probe is highly selective, which can clearly discriminate NO from other reactive species such as NO3–, NO, H2O2, •OH, GSH, cysteine, and ascorbic acid. Hence, this probe with good water-solubility demonstrates the ultra rapid, sensitive, and selective detection of endogenous NO, which is advantageous to investigate NO-related biological processes.
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