诱导多能干细胞
类有机物
生物
干细胞
人诱导多能干细胞
神经科学
再生(生物学)
定向微分
细胞分化
再生医学
计算生物学
细胞生物学
胚胎干细胞
遗传学
基因
作者
Ruobing Wang,Katie B. McCauley,Darrell N. Kotton,Finn Hawkins
标识
DOI:10.1016/bs.mcb.2020.03.008
摘要
There was significant progress over the last decade in the ability to generate induced pluripotent stem cell (iPSC)-derived airway organoids. We and others have developed step-wise, directed differentiation protocols to recapitulate the key milestones in human airway development, generating iPSC-derived airway organoids that possess the major human airway cell types. These organoids have already shown feasibility for genetic disease modeling. They have great future potential for modeling a wider spectrum of lung diseases, interrogating disease mechanisms, predicting personalized drug responses, studying developmental lung biology, and ultimately may serve as candidates for future cell-based therapies for lung regeneration and repair. Herein we detail a step-by-step laboratory protocol to generate human airway organoids.
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