In this paper, the effect of injected energy on low energy single longitudinal mode (SLM) pre-lase Q-switched is analyzed and the optimization procedure is shown in detail. Here, taking the Pr:YLF laser as an example of low energy laser, and the parameters of Pr:YLF laser by using pre-lase have been shown. Compared with normal Q-switched laser, the single pulse energy reaches 60.16% and the pulse width exceeds 39.73% when the same maximum energy is injected and SLM is achieved in pre-lase. The analysis results show that pre-lase is suitable for low energy laser to obtain SLM and there be an optimal performance to achieve the optimal energy output.