烟草花叶病毒
烟草
烟草
黄瓜花叶病毒
微生物学
病毒复制
植物病毒
水杨酸
病毒
病毒学
化学
生物
生物化学
基因
茄科
作者
Muhammad Adeel,Tahir Farooq,Jason C. White,Yi Hao,Zhao He,Yukui Rui
标识
DOI:10.1016/j.jhazmat.2020.124167
摘要
Although nanomaterials (NMs) may inhibit viral pathogens, the mechanisms governing plant–virus–nanomaterial interactions remain unknown. Nicotiana benthamiana plants were treated with nanoscale titanium dioxide (TiO2) and silver (Ag), C60 fullerenes, and carbon nanotubes (CNTs) at 100, 200 and 500 mg L−1 for a 21-day foliar exposure before inoculation with GFP-tagged tobacco mosaic virus (TMV). Plants treated with CNTs and C60 (200 mg L−1) exhibited normal phenotype and viral symptomology was not evident at 5 days post-infection. TiO2 and Ag failed to suppress viral infection. RT-qPCR analysis revealed that viral coat protein transcript abundance and GFP mRNA expression were reduced 74–81% upon CNTs and C60 treatment. TEM revealed that the chloroplast ultrastructure in carbon NM-treated plants was unaffected by TMV infection. Fluorescence measurement of CNTs and C60 (200 mg L−1) treated plants indicated photosynthesis equivalent to healthy controls. CNTs and C60 induced upregulation of the defense-related phytohormones abscisic acid and salicylic acid by 33–52%; the transcription of genes responsible for phytohormone biosynthesis was elevated by 94–104% in treated plants. Our findings demonstrate the protective role of carbon-based NMs, with suppression of TMV symptoms via hindered physical movement and viral replication. Given the lack of viral phytopathogen treatment options, this work represents a novel area of nano-enabled agriculture.
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