NADPH氧化酶
拟南芥
活性氧
生物
细胞生物学
磷酸化
拟南芥
丁香假单胞菌
激酶
第二信使系统
生物化学
信号转导
突变体
基因
作者
Sachie Kimura,Kerri Hunter,Lauri Vaahtera,Huy Cuong Tran,Matteo Citterico,Aleksia Vaattovaara,Anne Rokka,Sara C. Stolze,Anne Harzen,Lena Meißner,Maya Wilkens,Thorsten Hamann,Masatsugu Toyota,Hirofumi Nakagami,Michael Wrzaczek
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2020-02-07
卷期号:32 (4): 1063-1080
被引量:127
摘要
Reactive oxygen species (ROS) are important messengers in eukaryotic organisms, and their production is tightly controlled. Active extracellular ROS production by NADPH oxidases in plants is triggered by receptor-like protein kinase-dependent signaling networks. Here, we show that CYSTEINE-RICH RLK2 (CRK2) kinase activity is required for plant growth and CRK2 exists in a preformed complex with the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) in Arabidopsis (Arabidopsis thaliana). Functional CRK2 is required for the full elicitor-induced ROS burst, and consequently the crk2 mutant is impaired in defense against the bacterial pathogen Pseudomonas syringae pv tomato DC3000. Our work demonstrates that CRK2 regulates plant innate immunity. We identified in vitro CRK2-dependent phosphorylation sites in the C-terminal region of RBOHD. Phosphorylation of S703 RBOHD is enhanced upon flg22 treatment, and substitution of S703 with Ala reduced ROS production in Arabidopsis. Phylogenetic analysis suggests that phospho-sites in the C-terminal region of RBOHD are conserved throughout the plant lineage and between animals and plants. We propose that regulation of NADPH oxidase activity by phosphorylation of the C-terminal region might be an ancient mechanism and that CRK2 is an important element in regulating microbe-associated molecular pattern-triggered ROS production.
科研通智能强力驱动
Strongly Powered by AbleSci AI