荧光素酶
生物发光
荧光素
化学
生物搬运器
分子生物学
萤光素酶类
萤火虫协议
突变体
生物
生物化学
生物发光成像
体内
作者
Kenichi Noda,Hitoshi Goto,Yuji Murakami,Abo Bakr F. Ahmed,Akio Kuroda
标识
DOI:10.1016/j.ab.2009.10.025
摘要
The Limulus reaction is an application of the defense mechanism of horseshoe crab for endotoxin detection. Endotoxin is a component of the cell wall in the outer membrane of gram-negative bacteria, and causes fever or shock when it enters the human blood stream. For endotoxin detection, gel formation or turbidity of the coagulation factor chromogen or fluorescence-modified peptide is used. However, these conventional methods have problems with regard to their measurement time or sensitivity. We recently obtained a mutant firefly luciferase that has a luminescence intensity over 10-fold higher than that of the wild type. Therefore, we developed a new endotoxin detection method that combines the Limulus reaction and bioluminescence using mutant luciferase. The new method detects 0.0005 EU/ml of endotoxin within 15 min.
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