DPPH
特罗洛克
抗氧化剂
抗坏血酸
化学
Trolox当量抗氧化能力
芦丁
食品科学
类黄酮
儿茶素
多酚
传统医学
生物化学
医学
作者
Shang-Chih Lai,Yu-Ling Ho,Shun-Chieh Huang,Tai-Hung Huang,Zhen-Rung Lai,Chi-Rei Wu,Kuo-Yuan Lian,Yuan-Shiun Chang
标识
DOI:10.1142/s0192415x10007889
摘要
This study evaluated the antioxidant and antiproliferative activities of the crude extract and fractions of Desmodium triflorum (L.) DC. The total phenolic content, 1,1-diphenyl-2- picrylhydrazyl hydrate (DPPH) free radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), reducing power, total flavonoid content of D. triflorum were evaluated for the exploration of its antioxidant activities. Furthermore, its antiproliferative activities were investigated through the MTT method. It was compared with the antioxidant capacities of known antioxidants, including catechin, α-tocopherol, trolox and ascorbic acid. Among all fractions, ethyl acetate fraction was the most active in scavenging DPPH and TEAC radicals, of which 0.4 mg was equivalent to 186.6 ± 2.5 μg and 82.5 ± 2.1 μg of α-tocopherol and trolox respectively. The total phenolic and flavonoid contents of the crude extract were equivalent to 36.60 ± 0.1 mg catechin and 45.6 ± 0.6 mg rutin per gram respectively. In the reducing power assay, 1.25 mg of crude extract was similar to 61.2 ± 0.3 μg of ascorbic acid. For the assessment of the safety and toxicity of D. triflorum, LD 50 of the crude extract was greater than 10 g/kg when administered to mice through gastric intubation. The above experimental data indicated that D. triflorum was a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.
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