核糖核酸
生物
基因组
病毒学
计算生物学
RNA编辑
病毒
聚合酶
RNA依赖性RNA聚合酶
RNA病毒
非编码RNA
遗传学
基因
作者
Mark Mogler,Kurt I. Kamrud
标识
DOI:10.1586/14760584.2015.979798
摘要
The advent of reverse genetic approaches to manipulate the genomes of both positive (+) and negative (−) sense RNA viruses allowed researchers to harness these genomes for basic research. Manipulation of positive sense RNA virus genomes occurred first largely because infectious RNA could be transcribed directly from cDNA versions of the RNA genomes. Manipulation of negative strand RNA virus genomes rapidly followed as more sophisticated approaches to provide RNA-dependent RNA polymerase complexes coupled with negative-strand RNA templates were developed. These advances have driven an explosion of RNA virus vaccine vector development. That is, development of approaches to exploit the basic replication and expression strategies of RNA viruses to produce vaccine antigens that have been engineered into their genomes. This study has led to significant preclinical testing of many RNA virus vectors against a wide range of pathogens as well as cancer targets. Multiple RNA virus vectors have advanced through preclinical testing to human clinical evaluation. This review will focus on RNA virus vectors designed to express heterologous genes that are packaged into viral particles and have progressed to clinical testing.
科研通智能强力驱动
Strongly Powered by AbleSci AI