细胞生物学
四斯潘宁
罗亚
生物
内皮干细胞
细胞骨架
细胞粘附
血管生成
焦点粘着
肌动蛋白细胞骨架
血管通透性
细胞
信号转导
癌症研究
体外
生物化学
遗传学
内分泌学
作者
Feng Zhang,Jarett Michaelson,Simon Moshiach,Norman Sachs,Wenyuan Zhao,Yao Sun,Arnoud Sonnenberg,Jill M. Lahti,Hayden Huang,Xin A. Zhang
出处
期刊:Blood
[American Society of Hematology]
日期:2011-10-13
卷期号:118 (15): 4274-4284
被引量:44
标识
DOI:10.1182/blood-2011-03-339531
摘要
Tetraspanin CD151 is highly expressed in endothelial cells and regulates pathologic angiogenesis. However, the mechanism by which CD151 promotes vascular morphogenesis and whether CD151 engages other vascular functions are unclear. Here we report that CD151 is required for maintaining endothelial capillary-like structures formed in vitro and the integrity of endothelial cell-cell and cell-matrix contacts in vivo. In addition, vascular permeability is markedly enhanced in the absence of CD151. As a global regulator of endothelial cell-cell and cell-matrix adhesions, CD151 is needed for the optimal functions of various cell adhesion proteins. The loss of CD151 elevates actin cytoskeletal traction by up-regulating RhoA signaling and diminishes actin cortical meshwork by down-regulating Rac1 activity. The inhibition of RhoA or activation of cAMP signaling stabilizes CD151-silenced or -null endothelial structure in vascular morphogenesis. Together, our data demonstrate that CD151 maintains vascular stability by promoting endothelial cell adhesions, especially cell-cell adhesion, and confining cytoskeletal tension.
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