PI3K/AKT/mTOR通路
巨噬细胞极化
蛋白激酶B
体内
下调和上调
纤维化
药理学
白茅屬
肾
巨噬细胞
体外
癌症研究
化学
医学
生物
信号转导
生物化学
内科学
基因
生物技术
农学
作者
Xiaoyu Li,Xin Huang,Yuemin Feng,Yaqing Wang,Jibin Guan,Botian Deng,Qiuping Chen,Yanjing Wang,Yongming Chen,Jiahe Wang,Joe Yeong,Junfeng Hao
标识
DOI:10.1016/j.ejphar.2023.175771
摘要
Imperata cylindrica, a medicinal plant used in Traditional Chinese Medicine, has been used to treat chronic kidney disease. Extracts of I. cylindrica display anti-inflammatory, immunomodulatory, and anti-fibrotic properties. However, the active components of the extracts and their protective mechanisms have not been fully elucidated. In this study, we explored the ability of cylindrin, the main active compound extracted from I. cylindrica, to protect against renal fibrosis and to investigate the potential mechanisms involved. At high doses, cylindrin exerted protective effects against folic acid-induced kidney fibrosis in mice. Bioinformatic analysis predicted the LXR-α/PI3K/AKT pathway as a target of regulation by cylindrin. This was supported by our in vitro and in vivo results showing that cylindrin significantly downregulated the expression of LXR-α and phosphorylated PI3K/AKT in M2 macrophages and mouse renal tissues. Furthermore, high-dose cylindrin inhibited M2 polarization of IL-4-stimulated macrophages in vitro. Our results suggest that cylindrin alleviates renal fibrosis by attenuating M2 macrophage polarization through inhibition of the PI3K/AKT pathway via downregulation of LXR-α.
科研通智能强力驱动
Strongly Powered by AbleSci AI