核酸
清脆的
生物
核糖核酸
核酸检测
核酸定量
分子信标
分子生物学
计算生物学
生物化学
DNA
化学
基因
寡核苷酸
作者
Cheng Peng,Li Wang,Xiaoyun Chen,Xiaofu Wang,Lin Ding,Xiaoli Xu,Wei Wei,Lei Yang,Jian Wu,Meihao Sun,Junfeng Xu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2023-02-21
卷期号:8 (3): 1054-1063
被引量:21
标识
DOI:10.1021/acssensors.2c01955
摘要
Detecting short genetically modified (GM) nucleic acid fragments in GM crops and associated products is critically important for the global agriculture industry. Although nucleic acid amplification-based technologies have been widely used for genetically modified organism (GMO) detection, they still struggle to amplify and detect these ultra-short nucleic acid fragments in highly processed products. Here, we used a multiple-CRISPR-derived RNA (crRNA) strategy to detect ultra-short nucleic acid fragments. By combining confinement effects on local concentrations, an amplification-free CRISPR-based short nucleic acid (CRISPRsna) system was established to detect the cauliflower mosaic virus 35S promoter in GM samples. Moreover, we demonstrated assay sensitivity, specificity, and reliability by directly detecting nucleic acid samples from GM crops with a wide genomic range. The CRISPRsna assay avoided possible aerosol contamination from nucleic acid amplification and saved time due to an amplification-free approach. Given that our assay displayed distinct advantages over other technologies in detecting ultra-short nucleic acid fragments, it may have wide applications for detecting GM in highly processed products.
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