In Vitro Comparative Study of Calcium Hydroxyapatite (Stiim): Conventional Saline Dilution Versus Poly-Micronutrient Dilution

Calcium hydroxylapatite (CaHA) is widely used in aesthetics for its dual role as a dermal filler and biostimulator, promoting collagen and elastin synthesis. This study evaluates the comparative potential of CaHA in conventional saline dilution versus CaHA mixed with a poly-micronutrient solution (CaHA/PMN) to enhance its biostimulatory effects. In an in vitro model, primary human fibroblasts were treated with both formulations, and cell viability and gene expression of type I collagen, elastin, FOXO3, SIRT-1, and SIRT-3 were assessed. The results demonstrated that both CaHA and CaHA/PMN treatments significantly improved cellular responses compared to the control. CaHA/PMN outperformed conventional CaHA, with greater increases in cell viability, collagen synthesis, elastin synthesis, and the expression of SIRT-1, SIRT-3, and FOXO3. These findings suggest that poly-micronutrient-enriched solutions can enhance CaHA's regenerative potential, providing a promising approach for skin rejuvenation and elasticity improvement.