亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Low circulating tumor cell levels correlate with favorable outcomes and distinct biological features in multiple myeloma

多发性骨髓瘤 癌症研究 内科学 肿瘤科 生物 计算生物学 医学
作者
Ioannis V. Kostopoulos,Ioannis Ntanasis‐Stathopoulos,Pantelis Rousakis,Panagiotis Malandrakis,Chrysanthi Panteli,Evangelos Eleutherakis‐Papaiakovou,Nikolaos Angelis,Vasiliki Spiliopoulou,Panagiotis Bakouros,Rodanthi‐Eleni Syrigou,Georgia Dimitrakopoulou,Despina Fotiou,Magdalini Migkou,Nikolaos Kanellias,Nikolaos Paschalidis,Maria Gavriatopoulou,Efstathios Kastritis,Meletios Α. Dimopoulos,Ourania E. Tsitsilonis,Evangelos Terpos
出处
期刊:Research Square - Research Square
标识
DOI:10.21203/rs.3.rs-3978885/v1
摘要

Abstract Background There is growing interest in multiple myeloma (MM) circulating tumor cells (CTCs), but its rareness in peripheral blood (PB) and inconsistency in cutoffs question their clinical utility. Herein, we aimed at defining the optimal CTC cutoff using a highly sensitive approach. Moreover, we analyzed matched bone marrow (BM) and PB samples in order to highlight inconsistencies between the two sites and identify microenvironmental profiles associating with enhanced CTC dissemination. Methods Next-generation flow cytometry was performed for the detection of myeloma cells in matched BM and PB samples from 550 newly-diagnosed MM patients. Various multivariable regression models were applied for the detection of the optimal CTC clinical cutoff (median follow-up: 41 months). BM microenvironment was characterized by deep phenotyping using both CyTOF and various multiparametric flow cytometry panels. Results CTCs were detected in 90% of patients (median 0.01%, range: 0.0002% − 12.6%) and increased levels associated with adverse features. Unexpected correlations were observed between high CTC percentages and a diffused MRI pattern, a distinct BM composition characterized by altered B-cell differentiation together with an expansion of effector cells and tumor-associated macrophages, as well as a greater phenotypic dissimilarity between BM and PB tumor cells. Progression-free survival (PFS) and overall survival (OS) gradually worsened with each logarithmic increment of CTCs. Conversely, patients without CTCs showed unprecedented outcomes (5-year PFS and OS: 83% and 97%, respectively). A cutoff of 0.02% CTCs was independent of the ISS, LDH and cytogenetics in a multivariate analysis of risk factors for PFS and proved clinically relevant for both TE and TI patients. The 0.02% CTC cutoff synergized with the MGUS-like phenotype and the R-ISS for improved risk stratification. MRD-negativity was less frequent if CTCs were ≥ 0.02% at diagnosis, but whenever achieved, the poor prognosis of these patients was abrogated. Conclusions This study shows the clinical utility of CTC assessment in MM and provides evidence towards a consensus cutoff for risk stratification. The liquid biopsy is more informative of the entire tumor burden than a single-spot BM aspiration; hence, CTC analysis may serve as the new hallmark for the real-time evaluation of a patient’s disease status.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
4秒前
量子星尘发布了新的文献求助10
11秒前
33秒前
37秒前
Dandelion完成签到,获得积分10
45秒前
1分钟前
完美世界应助nsc采纳,获得10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
所所应助nsc采纳,获得10
1分钟前
小蘑菇应助nsc采纳,获得50
1分钟前
Ava应助nsc采纳,获得10
1分钟前
隐形曼青应助nsc采纳,获得10
1分钟前
慕青应助nsc采纳,获得10
1分钟前
脑洞疼应助nsc采纳,获得10
1分钟前
善学以致用应助nsc采纳,获得10
1分钟前
ding应助nsc采纳,获得30
1分钟前
Hello应助nsc采纳,获得10
1分钟前
烟花应助nsc采纳,获得10
1分钟前
1分钟前
1分钟前
漫步随心完成签到,获得积分20
2分钟前
NexusExplorer应助科研通管家采纳,获得10
2分钟前
2分钟前
今后应助nsc采纳,获得10
2分钟前
bkagyin应助nsc采纳,获得10
2分钟前
小二郎应助nsc采纳,获得10
2分钟前
Jasper应助nsc采纳,获得10
2分钟前
李爱国应助nsc采纳,获得10
2分钟前
脑洞疼应助nsc采纳,获得10
2分钟前
慕青应助nsc采纳,获得10
2分钟前
天天快乐应助nsc采纳,获得10
2分钟前
Akim应助nsc采纳,获得10
2分钟前
充电宝应助nsc采纳,获得10
2分钟前
量子星尘发布了新的文献求助30
3分钟前
3分钟前
3分钟前
3分钟前
3分钟前
3分钟前
3分钟前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
T/CIET 1202-2025 可吸收再生氧化纤维素止血材料 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3957061
求助须知:如何正确求助?哪些是违规求助? 3503084
关于积分的说明 11111255
捐赠科研通 3234121
什么是DOI,文献DOI怎么找? 1787751
邀请新用户注册赠送积分活动 870762
科研通“疑难数据库(出版商)”最低求助积分说明 802264