抄写(语言学)
G-四倍体
基因
生物
细胞生物学
分子生物学
化学
DNA
遗传学
语言学
哲学
作者
Jihee Hwang,Bradleigh Palmer,Sua Myong
出处
期刊:Methods in Enzymology
日期:2024-01-01
卷期号:: 71-88
被引量:1
标识
DOI:10.1016/bs.mie.2024.01.001
摘要
Potential G-quadruplex forming sequences (PQS) are enriched in cancer-related genes and immunoglobulin class-switch recombination. They are prevalent in the 5'UTR of transcriptionally active genes, thereby contributing to the regulation of gene expression. We and others previously demonstrated that the PQS located in the non-template strand leads to an R-loop formation followed by a G-quadruplex (G4) formation during transcription. These structural changes increase mRNA production. Here, we present how single-molecule technique was used to observe cotranscriptional G4 and R-loop formation and to examine the impact on transcription, particularly for the initiation and elongation stages.
科研通智能强力驱动
Strongly Powered by AbleSci AI