An integrated sample-to-answer graphene microchip for ultrafast detection of multiple microorganisms

石墨烯 微生物 样品(材料) 超短脉冲 纳米技术 材料科学 生物 化学 物理 色谱法 光学 细菌 遗传学 激光器
作者
Quanying Fu,Shaoqiong Feng,Zihan Xiao,Kai Gong,Xueyuan Pang,Yang Yue,Chixing Zhou,Haidong Liu,Jiasi Wang,Jianhua Zhou
出处
期刊:Sensors and Actuators B-chemical [Elsevier]
卷期号:409: 135584-135584
标识
DOI:10.1016/j.snb.2024.135584
摘要

The integrated detection of nucleic acids is a molecular biological technology integrating the entire process of nucleic acid detection into a "sample-to-answer" system, which is widely used in microbial identification and disease diagnosis. Although the integrated detection of nucleic acids has the advantages of fully-enclosed system and simple operation, it still faces challenges like dependence on bulky equipment, complex design, and long detection time. To address these challenges, we have proposed and fabricated an integrated graphene microchip for ultra-fast detection of multiple microorganisms. This microchip is composed of two main regions: the electrolysis-extraction region and the isothermal amplification-detection region. In the electrolysis-extraction region, the microchip was equipped with the micron-structured graphene interdigital electrode and a regular battery for power supply, and the nucleic acids of H. pylori were extracted only in 1.0 s by electrolysis. Then in the isothermal amplification-detection region, the nucleic acids of H. pylori were detected in less than 9 min at a concentration of 10 CFU/µL. As a result, the detection time of the whole process were controlled in merely 9 min. In addition, this integrated graphene microchip has three parallel channels for simultaneous detection of oral microorganisms, e.g., C. albicans, S. aureus and H. pylori. These characteristics make our integrated graphene microchip promising to contribute to the development in integrated technology of nucleic acid detection and be widely used at various medical structures or on-site environments such as home testing.
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