Assessing Susceptibility of Grapevine Cultivars to Latent Plasmopara viticola Infections Using Molecular Disease Index

葡萄疟原虫 生物 栽培 霜霉病 植物抗病性 病菌 园艺 接种 植物 微生物学 遗传学 基因
作者
Lujia Yang,Bingyao Chu,Jie Deng,Zhenguo Shen,Qiuyu Sun,Xuan Lv,Jiasui Zhan,Zhanhong Ma
出处
期刊:Phytopathology [American Phytopathological Society]
标识
DOI:10.1094/phyto-10-23-0409-kc
摘要

Traditional assessments of grapevine susceptibility to grapevine downy mildew (GDM) caused by Plasmopara viticola rely on the visual evaluation of leaf symptoms. In this study, we used a well-established quantitative real-time PCR TaqMan assay (real-time PCR) to quantify the number of P. viticola infecting 12 grapevine cultivars under controlled conditions. The molecular disease index (MDI), derived from molecular detection methods, reflects the relative abundance of pathogens in plant tissues during the latent infection phase. Our application of MDI revealed a progressive increase in latent P. viticola levels over time, indicating dynamic levels of latent P. viticola infection across the inoculation processes. We calculated the area under the disease progression curve in terms of MDI (AUDPCMDI) to evaluate the susceptibility of each cultivar to GDM. Cultivars with lower AUDPCMDI values consistently exhibited reduced pathogen establishment, suggesting higher levels of innate resistance. Correlation analysis revealed a significant correlation between the visual disease index (DI) and the AUDPCMDI values (r = 0.790, P = 0.002), indicating that higher levels of latent P. viticola infections were associated with higher disease severity. Grapevine cultivars were clustered into distinct groupings, indicating variability in their susceptibility to the pathogen. Cultivars with similar levels of susceptibility were grouped, highlighting that the real-time PCR assay used in this study represents a robust, rapid, and standardized method for quantifying pathogens, which significantly improves the efficiency of evaluating the susceptibility of grapevine cultivars to GDM This quantitative protocol provides practical guidelines for selecting resistant cultivars and implementing effective disease management strategies.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
赎罪完成签到 ,获得积分10
1秒前
Qsss完成签到,获得积分10
2秒前
ya发布了新的文献求助10
2秒前
2秒前
练得身形似鹤形完成签到 ,获得积分10
3秒前
3秒前
3秒前
4秒前
鹿港猫妖完成签到,获得积分20
5秒前
chen完成签到,获得积分10
5秒前
6秒前
阿湫发布了新的文献求助10
6秒前
6秒前
7秒前
崔悦欣发布了新的文献求助10
7秒前
希望天下0贩的0应助kk子采纳,获得10
8秒前
丰富的不惜完成签到,获得积分10
10秒前
票子发布了新的文献求助10
10秒前
华仔应助魏莱采纳,获得10
10秒前
F123发布了新的文献求助10
11秒前
shen发布了新的文献求助10
12秒前
12秒前
苗觉觉完成签到,获得积分10
13秒前
帕金森完成签到,获得积分10
14秒前
活力的妙芙完成签到,获得积分10
14秒前
赘婿应助疯子魔煞采纳,获得10
15秒前
橙橙橙完成签到,获得积分10
15秒前
15秒前
你好CDY完成签到,获得积分10
16秒前
16秒前
16秒前
万能图书馆应助希尔伯特采纳,获得10
16秒前
我是老大应助123采纳,获得10
17秒前
芒果小鹌鹑完成签到,获得积分10
18秒前
上官若男应助F123采纳,获得10
19秒前
Singularity应助邢文瑞采纳,获得10
20秒前
c123发布了新的文献求助10
21秒前
嘉嘉琦发布了新的文献求助10
22秒前
wanci应助QWDSA采纳,获得10
24秒前
24秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038446
求助须知:如何正确求助?哪些是违规求助? 3576149
关于积分的说明 11374627
捐赠科研通 3305875
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892680
科研通“疑难数据库(出版商)”最低求助积分说明 815048