蛋白激酶B
细胞凋亡
PI3K/AKT/mTOR通路
MAPK/ERK通路
化学
细胞
细胞生物学
信号转导
信号通路
癌症研究
人参皂甙
细胞生长
生物
生物化学
医学
人参
病理
替代医学
作者
Beibei Geng,Man Zhao,Cheng Wang,Tian Zhong,Chaoyan Kang,Zizhen Wang,Hanxin Wu,Ting Xia
标识
DOI:10.1080/14786419.2024.2440537
摘要
T-cell acute lymphoblastic leukaemia (T-ALL) is a common childhood malignant tumour, which has poor prognosis and high recurrence rate. Ginsenoside Rh2 (GRh2), a bioactive ingredient of Panax ginseng has significant anti-tumour effect. In this study, we found that gene expressions of Jurkat cells were significantly changed in the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signalling pathways after 35 µm GRh2 treatment, involving in JUN, PIEN, AKT3 and MAPK8IP2. Target proteins including PI3K, AKT, ASK, caspase 8 and caspase 9 were bind tightly with GRh2 by molecular docking. Moreover, the protein expression ratios of p-PI3K/PI3K and p-AKT/AKT were significantly reduced, and the expression ratios of p-ASK1/ASK1, p-JNK/JNK and p-c-JUN/c-JUN, Bax/Bcl-2, and the levels of cleaved caspase 8, 9, 3 were increased significantly in GRh2-treated Jurkat cells. The results imply that GRh2 induced T-ALL apoptosis by activating the MAPK pathway and inhibiting the PI3K-AKT pathway.
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