The molecule events expression of TGF-β/Smad signaling pathway in morphological and structural developmental characteristics of gonads in goose embryos

SMAD公司 胚胎 细胞生物学 转化生长因子 生物 信号转导 原肠化 胚胎发生 男科 医学 生态学
作者
Xianou Fu,Qiuyuan Liu,Yupu Song,Yudong Wang,Yuxuan Zhou,Sihui Wang,Tuoya Liu,Fangming Zhuang,Jingtao Hu,Yongfeng Sun
出处
期刊:Poultry Science [Elsevier BV]
卷期号:104 (2): 104808-104808
标识
DOI:10.1016/j.psj.2025.104808
摘要

China is the largest producer and consumer of geese with significant social and economic value in agriculture. The Jilin White Goose, known for its excellent egg-laying and reproductive characteristics, is a prominent breeding breed in the northeast of China widely used for cross-breeding.Gonad development is a complex process, which will differentiate into testes or ovaries, thus affecting sex determination. Since the level of gonadal development in geese of different sexes is also related to their productivity, the study of gonadal function in geese is of great theoretical and practical importance. In this study, we used the embryonic gonads of Jilin white geese as the research object to reveal the morphologic and histologic developmental characteristics and process of the gonads of goose embryos from the 9th day to the 28th day and to investigate the mechanisms regulating the differentiation of the gonads. The results of ALP-PAS staining by histomorphological observations showed that the primordial germ cells in the female gonads were fully differentiated at embryonic day 19 (E19) and in the male gonads at embryonic day 18 (E18).Previous studies indicate that the TGF-β /Smad signaling pathway is crucial for sex differentiation and gamete formation in animals. In our study, we quantitatively analyzed key genes and proteins in this pathway within goose embryos. We found that TGF-βRI/II receptors and Smad2/3 are involved in gonadal development early in differentiation. Smad2 protein levels peaked at E18 in male gonads and E19 in female gonads, while Smad3 peaked at E10 for both. It has been suggested that there is a novel interaction between TGF-β and Wnt/β-catenin signaling pathway. In this study, the expression of LEF1, a protein downstream of the Wnt/β-catenin signaling pathway, peaked at E19 in the female gonads and at E18 in the male gonads, and the protein expression level of β-catenin was significantly increased in the female gonads at E19 and E28 and peaked at E28 in the male gonads. β-catenin expression levels were significantly higher in the male gonads than in the other periods and reached a minimum at E28. We injected the TMAO drug, which activates the TGF-β /Smad pathway, into goose embryos using the embryo injection technique to study how it affects gene and protein expression in this signaling pathway. We found that in female gonads, the levels of Smad2/3 proteins were lower than in the control group at E18, but higher than the control group at E19. In male gonads, the results for Smad3 proteins matched those of the females, while the results for Smad2 proteins were the opposite. This shows that TMAO activation of the TGF-β /Smad signaling pathway may impact gonadal development in goose embryos. In conclusion, this study suggest that the TGF-β /Smad and Wnt signaling pathways may enhance gonadal tissue development in goose embryos. The study identified key time points in gonadogenesis, providing a new theoretical basis for further research on the molecular mechanisms of gonadal development.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
eosin发布了新的文献求助10
2秒前
4秒前
5秒前
研友_VZG7GZ应助云叶采纳,获得10
5秒前
彭于彦祖应助whisper采纳,获得30
7秒前
vic303发布了新的文献求助10
8秒前
9秒前
欢快的芹菜完成签到,获得积分10
9秒前
大个应助Abx采纳,获得10
11秒前
11秒前
12秒前
小于发布了新的文献求助10
12秒前
隐形曼青应助yxy303256651采纳,获得10
13秒前
13秒前
Stevielau发布了新的文献求助10
13秒前
13秒前
领导范儿应助河北采纳,获得100
14秒前
木九发布了新的文献求助30
16秒前
17秒前
nesire发布了新的文献求助10
17秒前
yuyuan完成签到,获得积分10
17秒前
Hhhh给Hhhh的求助进行了留言
19秒前
柚子君关注了科研通微信公众号
19秒前
20秒前
BOB完成签到,获得积分10
21秒前
21秒前
22秒前
22秒前
leichun完成签到,获得积分10
22秒前
dong应助杨憨憨采纳,获得10
22秒前
情怀应助wangrswjx采纳,获得10
23秒前
慈祥的发卡完成签到 ,获得积分10
25秒前
Abx发布了新的文献求助10
25秒前
kento发布了新的文献求助10
25秒前
26秒前
27秒前
wx发布了新的文献求助10
27秒前
乐观小之发布了新的文献求助700
27秒前
情怀应助七一同学采纳,获得10
27秒前
Sun关闭了Sun文献求助
28秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3972247
求助须知:如何正确求助?哪些是违规求助? 3516737
关于积分的说明 11184569
捐赠科研通 3252221
什么是DOI,文献DOI怎么找? 1796273
邀请新用户注册赠送积分活动 876339
科研通“疑难数据库(出版商)”最低求助积分说明 805483