Screening and Exploring the Application of the Multifunctional Antioxidant Peptides MSWLC and TSWLC

Abstract In this study, an antioxidant pentapeptide library is created based on antioxidant characteristics. The peptides are then purified and separated using liquid chromatography/mass spectrometry (LC/MS) and time‐of‐flight mass spectrometry (TOF). Chemical evaluations identify four peptides with excellent antioxidant activity. The four peptides undergo biocompatibility testing with L‐929, NIH 3T3, and Hep‐G2 cells. A model of hydrogen peroxide‐induced cellular damage in G2 cells shows the peptides' protective and reparative effects against oxidative damage. Two peptides, MSWLC and TSWLC, which perform best overall, are chosen for further analysis. To explore the peptides' potential multifunctionality, acute liver inflammation, keratitis, and aging models are established in mice. MSWLC and TSWLC demonstrate anti‐inflammatory and anti‐aging properties. An antioxidant emulsion prepared by emulsification is found to be non‐irritant in a mouse skin irritation test. In a mouse model exposed to ultraviolet radiation, the sunscreen exhibits excellent UV protection and antioxidant effects. These peptides possess potent antioxidant properties and multifunctionality, indicating broad application potential.