丙炔基转移酶
韧皮部
化学
立体化学
突变体
IC50型
生物化学
酶
基因
体外
预酸化
作者
Yanzhi Yang,Linlan Tao,Yunyun Li,Ying Wu,Qianqian Ran,Dan Li,Shu‐Ming Li,Xia Yu,Chun‐Mao Yuan,Kang Zhou
标识
DOI:10.1021/acs.jafc.4c00928
摘要
Phloretin is widely found in fruit and shows various biological activities. Here, we demonstrate the dimethylallylation, geranylation, and farnesylation, particularly the first dimethylallylation at the nonaromatic carbon of phloretin (1) by the fungal prenyltransferase AnaPT and its mutants. F265 was identified as a key amino acid residue related to dimethylallylation at the nonaromatic carbon of phloretin. Mutants AnaPT_F265D, AnaPT_F265G, AnaPT_F265P, AnaPT_F265C, and AnaPT_F265Y were discovered to generally increase prenylation activity toward 1. AnaPT_F265G catalyzes the O-geranylation selectively at the C-2′ hydroxyl group, which involves an intramolecular hydrogen bond with the carbonyl group of 1. Seven products, 1D5, 1D7–1D9, 1G2, 1G4, and 1F2, have not been reported prior to this study. Twelve compounds, 1D3–1D9, 1G1–1G3, and 1F1–1F2, exhibited potential inhibitory effects on α-glucosidase with IC50 values ranging from 11.45 ± 0.87 to 193.80 ± 6.52 μg/mL. Among them, 1G1 with an IC50 value of 11.45 ± 0.87 μg/mL was the most potential α-glucosidase inhibitor, which is about 30 times stronger than the positive control acarbose with an IC50 value of 346.63 ± 15.65 μg/mL.
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