Integrated serum pharmacochemistry with network pharmacology and pharmacological validation to elucidate the mechanism of yiqitongmai decoction (YQTMD) against myocardial infarction

汤剂 药理学 心肌梗塞 医学 传统医学 生药学 机制(生物学) 口服 生物活性 化学 心脏病学 体外 生物化学 认识论 哲学
作者
Hao Yu,Ning Wang,Xuerong Zhao,Lan Han,Jinyong Peng
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:331: 118329-118329 被引量:1
标识
DOI:10.1016/j.jep.2024.118329
摘要

Yiqitongmai decoction (YQTMD), a classic TCM, has been widely used in clinical treatment for MI. However, it is still difficult to clarify the potential active compounds and pharmacological mechanisms of it in treating MI. To explore the active ingredients, pharmacological effects, potential targets and mechanisms of YQTMD against MI. Serum pharmacochemistry by UPLC-MS/MS was applied to analyze the phytochemical components in serum from YQTMD. These components were then used to predict the potential targets using network pharmacology approach and molecular dynamics simulations, and then the protective effect of them on H9c2 cells following hypoxic conditions was assessed. Afterwards, the pharmacological effects of YQTMD on MI in mice were tested by determining electrocardiogram (ECG), echocardiography, cardiac biomarkers, oxidative stress, inflammation and pathophysiological changes. The protein levels involving STAT3 signal were detected using western blot and immunofluorescence assays. Furthermore, STAT3 inhibitor Sttatic was employed to further elucidate the underlying mechanisms. Totally, 26 compounds derived from YQTMD were identified in mice serum, and 201 genes associated with the compounds were collected. The compounds including safflomin A, ferulic acid, gypenoside XVII, ginsenoside Rg1 and glycyrrhizic acid were identified as the critical compounds of YQTMD to regulate STAT3 pathway. In vitro, compounds combination significantly enhanced the viability of H9c2 cells and reduced ROS level compared to model cells. The in vivo results showed that YQTMD effectively reduced myocardial injury, as evidenced by the decreased serum cardiac injury markers, reduction in the size of myocardial infarct, restoration of abnormal alterations in ECG and decrease in cardiomyocyte apoptosis. Additionally, YQTMD attenuated MI-induced cardiac dysfunction, alleviated pathological changes, reduced MDA levels, and enhanced SOD and GSH levels compared with model mice. Significantly, the levels of IL-6, IL-1β, and TNF-α were observed to decrease in the YQTMD group. The expression levels of key proteins (p-STAT3, HIF-1α, NOX2, TLR5 and Caspase3) in STAT3 pathway were also regulated by YQTMD. However, the cardioprotective effects of YQTMD on MI were attenuated by STAT3 inhibitor Sttatic. This study investigated the active ingredients and potential mechanisms of YQTMD for MI treatment based on serum pharmacochemistry and network pharmacology approaches, revealing that YQTMD exerts its therapeutic effects on MI by alleviating oxidative stress, inflammation and apoptosis through adjusting STAT3 signaling pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
888完成签到,获得积分10
刚刚
刚刚
xcwy完成签到,获得积分10
刚刚
yu发布了新的文献求助10
刚刚
刚刚
wwww完成签到 ,获得积分10
1秒前
1秒前
糊涂的麦片完成签到,获得积分10
2秒前
王王发布了新的文献求助10
2秒前
SYLH应助Mr.Su采纳,获得10
2秒前
Qing完成签到,获得积分10
2秒前
2秒前
852应助炙心采纳,获得10
2秒前
2秒前
3秒前
5433发布了新的文献求助10
3秒前
3秒前
小马甲应助予夏书采纳,获得30
4秒前
4秒前
搞笑5次完成签到,获得积分10
5秒前
cjz完成签到,获得积分20
5秒前
5秒前
YY发布了新的文献求助10
5秒前
David完成签到,获得积分10
5秒前
辣子鸡发布了新的文献求助10
5秒前
wang_qi发布了新的文献求助10
6秒前
6秒前
Ao关注了科研通微信公众号
6秒前
小王完成签到,获得积分10
7秒前
思源应助xcwy采纳,获得10
7秒前
cjz发布了新的文献求助10
7秒前
炙心完成签到,获得积分10
7秒前
8秒前
猪猪hero发布了新的文献求助10
8秒前
陶醉的夏槐完成签到,获得积分10
9秒前
鳗鱼绿蝶完成签到,获得积分10
9秒前
852应助吴小台呀采纳,获得10
9秒前
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3970632
求助须知:如何正确求助?哪些是违规求助? 3515261
关于积分的说明 11177794
捐赠科研通 3250448
什么是DOI,文献DOI怎么找? 1795314
邀请新用户注册赠送积分活动 875781
科研通“疑难数据库(出版商)”最低求助积分说明 805073