乙型肝炎表面抗原
乙型肝炎病毒
神秘的
基因型
病毒学
突变体
乙型肝炎
突变
生物
免疫学
医学
基因
病毒
遗传学
病理
替代医学
作者
Huizhen Sun,Le Chang,Ying Yan,Huimin Ji,Xinyi Jiang,Shi Song,Yingzi Xiao,Zhuoqun Lu,Lunan Wang
标识
DOI:10.1016/j.antiviral.2022.105448
摘要
Occult hepatitis B virus (HBV) infection (OBI) has non-negligible clinical significance, but the mechanism of its occurrence remains unclear. Growing evidence suggests that mutations in the pre-S region of HBV genome may be associated with the occurrence of OBI. However, the role of pre-S mutations in OBI and its molecular mechanism was not fully understand. Here, the pre-S sequences from 307 OBI blood donors and 293 hepatitis B surface protein (HBsAg)-positive blood donors were obtained, and we observed a higher frequency of naturally occurring pre-S mutations in OBI donors infected with genotype B/C HBV than in HBsAg-positive donors, suggesting their potential positive role in OBI. In both genotype B and C, several pre-S mutants resulted in markedly reduced HBsAg production in vitro. In particular, the T68I, S78N and N98T mutants of genotype B were proven to significantly decrease the HBsAg synthesis by affecting the pre-S2/S promoter activity, and thereby promoting the occurrence of OBI.
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