蛋白酶
两亲性
化学
生物传感器
分散性
组合化学
肽
自组装
纳米技术
连接器
生物物理学
材料科学
生物化学
酶
有机化学
生物
聚合物
操作系统
计算机科学
共聚物
作者
Zhicheng Jin,Yi Li,Ke Li,Jiajing Zhou,Justin Yeung,Chuxuan Ling,Wonjun Yim,Tengyu He,Yong Cheng,Ming Xu,Matthew N Creyer,Yu-Ci Chang,Pavla Fajtová,Maurice Retout,Baiyan Qi,Shuzhou Li,Anthony J. O'Donoghue,Jesse V. Jokerst
标识
DOI:10.1002/anie.202214394
摘要
Aromatic interactions are commonly involved in the assembly of naturally occurring building blocks, and these interactions can be replicated in an artificial setting to produce functional materials. Here we describe a colorimetric biosensor using co-assembly experiments with plasmonic gold and surfactant-like peptides (SLPs) spanning a wide range of aromatic residues, polar stretches, and interfacial affinities. The SLPs programmed in DDD-(ZZ)x -FFPC self-assemble into higher-order structures in response to a protease and subsequently modulate the colloidal dispersity of gold leading to a colorimetric readout. Results show the strong aggregation propensity of the FFPC tail without polar DDD head. The SLPs were specific to the target protease, i.e., Mpro , a biomarker for SARS-CoV-2. This system is a simple and visual tool that senses Mpro in phosphate buffer, exhaled breath condensate, and saliva with detection limits of 15.7, 20.8, and 26.1 nM, respectively. These results may have value in designing other protease testing methods.
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