体内
糖基化
聚糖
化学
体外
原位
A549电池
生物化学
细胞生物学
癌症研究
生物
糖蛋白
生物技术
有机化学
作者
Dongxia Chen,Yaying Lin,Yifan Fan,Lingxuan Li,Chenlei Tan,Junjie Wang,Hongyu Lin,Jinhao Gao
标识
DOI:10.1002/anie.202313753
摘要
Abstract The abnormality in the glycosylation of surface proteins is critical for the growth and metastasis of tumors and their capacity for immunosuppression and drug resistance. This anomaly offers an entry point for real‐time analysis on glycosylation fluctuations. In this study, we report a strategy, glycan metabolic fluorine labeling (MEFLA), for selectively tagging glycans of tumor cells. As a proof of concept, we synthesized two fluorinated unnatural monosaccharides with distinctive 19 F chemical shifts (Ac 4 ManNTfe and Ac 4 GalNTfa). These two probes could undergo selective uptake by tumor cells and subsequent incorporation into surface glycans. This approach enables efficient and specific 19 F labeling of tumor cells, which permits in vivo tracking of tumor cells and in situ assessment of glycosylation changes by 19 F MRI. The efficiency and specificity of our probes for labeling tumor cells were verified in vitro with A549 cells. The feasibility of our method was further validated with in vivo experiments on A549 tumor‐bearing mice. Moreover, the capacity of our approach for assessing glycosylation changes of tumor cells was illustrated both in vitro and in vivo. Our studies provide a promising means for visualizing tumor cells in vivo and assessing their glycosylation variations in situ through targeted multiplexed 19 F MRI.
科研通智能强力驱动
Strongly Powered by AbleSci AI