质量细胞仪
多路复用
细胞仪
肝细胞癌
免疫系统
流式细胞术
生物
计算生物学
癌症研究
生物信息学
免疫学
基因
生物化学
表型
作者
Yongtao Ji,Dongpeng Sun,Yaxing Zhao,Jiao Tang,Jianghui Tang,Juan Song,Junlei Zhang,Xun Wang,Wang Shao,Dong Chen,Jianpeng Sheng,Qi Zhang
出处
期刊:Nano Today
[Elsevier]
日期:2023-07-27
卷期号:52: 101940-101940
被引量:4
标识
DOI:10.1016/j.nantod.2023.101940
摘要
Early diagnosis of hepatocellular carcinoma (HCC), a highly malignant disease, is crucial for improving patient prognosis. Liquid biopsy has emerged as a promising approach for cancer detection, especially peripheral immunity profiling using mass cytometry time-of-flight (CyTOF). While the limitied throughput and high cost hamper its widespread application. To address these challenges, we developed an automated micro-droplet printing platform to achieve high-throughput mass cytometry barcoding (MCB) strategy. Utilizing a poly (methyl methacrylate) (PMMA) plane substrate, we established a multiplex barcoding checkboard in binary labeling strategy through "plate covering". To validate the utility of our system, we applied the multiplex barcoding system to label human peripheral blood samples from two cohorts consisting of 16 and 64 samples respectively, while successfully recapitulating the immune features associated with HCC. The ability of high-throughput barcoding samples empowers the application of CyTOF for large-scale HCC detection and other potential uses.
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