Regulatory T-Cell Phenotyping Using CyTOF

AbstractRegulatory T cells are an important component of the immune system that plays a key role in maintaining homeostasis. Identification of distinct regulatory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapter describes a mass cytometry approach for phenotypic characterization of regulatory T cells and determination of their master transcription factor Foxp3.Key wordsRegulatory T cellsTregFoxp3Mass cytometryCyTOFphenotypingimmunophenotypingSingle-cell analysis