热稳定性
聚对苯二甲酸乙二醇酯
解聚
降级(电信)
材料科学
生物降解
聚乙烯
化学工程
环境污染
高分子化学
化学
有机化学
酶
复合材料
环境科学
计算机科学
环境保护
工程类
电信
作者
Qingdian Yin,Shengping You,Jiaxing Zhang,Wei Qi,Rongxin Su
标识
DOI:10.1016/j.biortech.2022.128026
摘要
The serious environmental pollution that came up with the continuously growing demand for polyethylene terephthalate (PET) has attracted global concern. The IsPETase which has shown the highest PET degradation activity under ambient temperature is a promising enzyme for PET biodegradation, while poor thermostability limited its practical application. Herein, an electrostatic interaction-based strategy was applied for rational design of IsPETase towards enhanced thermostability. The IsPETaseI139R variant displayed the highest Tm value of 56.4 °C and 3.6-times higher PET degradation activity. Molecular simulations demonstrated that the introduction of salt bridges stabilized the local structures, resulting in robust thermostability. Meanwhile, the IsPETaseS92K/D157E/R251A not only exhibited higher thermostability but also showed a 1.74-fold kcat increase towards mono-(2-hydroxyethyl) terephthalate, which ultimately achieved PET depolymerization to complete monomer TPA. Collectively, the electrostatic interaction-based strategy, together with the derived IsPETase variants, could help promote the bio-recycle of PET, reducing the severe global burden of PET waste.
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