连接蛋白
蛋白激酶B
癌症研究
LY294002型
光动力疗法
结直肠癌
PI3K/AKT/mTOR通路
化学
医学
癌症
生物
内科学
细胞生物学
磷酸化
信号转导
缝隙连接
细胞内
有机化学
作者
Yijia Wang,Lankai Chen,Sizhen Lai,Yanfei Liu,Ben Yi,Siwei Zhu,Xia Hu,Qinghuai Zhang,Chunze Zhang
标识
DOI:10.1016/j.pdpdt.2022.103040
摘要
Photodynamic therapy could be one approach to treat colorectal cancer though resistance leads to failure of therapy. Akt activation is a cellular survival response to photodynamic therapy and is also a reason for resistance. Thus, inhibition of Akt is a strategy to decrease resistance. Akt interacts with connexin 43, another protein involved in photodynamic therapy resistance. Connexin 43 is widely expressed in different human tissues and has a complex role in tumor development. However, the mechanism of inhibition of Akt by connexin 43 that sensitizes colorectal cancer cells to photodynamic therapy needs further investigation. In this study, two colorectal cancer cells with low phosphorylated connexin 43 level were used to explore this mechanism. LY294002 was used as an Akt inhibitor, and connexin 43-pCMV3 was transfected into cells to increase connexin 43 expression. Akt and connexin 43 inhibit each other in both colorectal cancer cell lines. In vitro and in vivo experiments showed that LY294002 and connexin 43 transfection sensitized cells to hematoporphyrin-Photodynamic therapy. LY294002 increased the sensitivity of cells to photodynamic therapy with a pronounced effect in cells with high expression levels of connexin 43. Connexin 43 should be considered an important factor in increasing the phototoxicity of photodynamic therapy in colorectal cancer through Akt inhibition.
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