Study on quantitative analysis of acephate by ATR-FTIR spectroscopy

In situ attenuated total reflection infrared spectroscopy was used to detect acephate at different times.It indicated that acephate was relatively stable and hardly volatile. There were two methods of quantitative analysis of acephate by Infrared Spectroscopy,one used the characteristic absorption peak height at 1 697 cm- 1,1 462 cm- 1,1 216 cm- 1and 1 038 cm- 1to concentration making standard curve and its linear regression coefficient R were 0. 996 4,0. 996 3,0. 996 6 and 0. 996 7,the other used those peak integral area to concentration for standard curve and the linear regression coefficient R were 0. 992 2,0. 990 5,0. 990 2 and 0. 992 9. The mean value of the former was 0. 996 5 while the latter was 0. 991 5. The result showed that using absorption peak height to concentration for standard curve is better in linear than the one made by peak integral area to concentration. Acephate within 1. 0 ~ 8. 0 μg·mL- 1conformed to the linear relationship.