体内
细胞凋亡
体外
流式细胞术
肝细胞癌
细胞生长
免疫细胞化学
MTT法
分子生物学
医学
癌症研究
生物
病理
生物化学
生物技术
出处
期刊:Chinese clinical oncology
[AME Publishing Company]
日期:2011-01-01
摘要
Objective To investigate the inhibitory function of oligochitosan on hepatocellular carcinoma cells in vivo and in vitro.Methods In experiment in vitro,MTT assay was applied for evaluation of proliferation of SMMC-7721 cells treated by oligochitosan.Apoptotic cells were detected by flow cytometry and RT-PCR was used for the expression of Bcl-2.In experiment in vivo,we observed the effect of oligochitosan on the growth of H22 cell subcutaneous transplanting tumor in mice.Immunocytochemistry was utilized to measure the expression of Bcl-2 in the tumor tissues.Results Experiment in vitro showed oligochitosan inhibited SMMC-7721 cell proliferation and induced the apoptosis of them.This effect increased with the increasing dosage.The tumor inhibiting rate was 23.82% after treated with 4mg/ml of oligochitosan and the apoptosis rate was 15.28% with 2mg/ml of oligochitosan.Compared with those of the control group,the inhibitory effect was significant(P0.01).The expression of Bcl-2 mRNA in SMMC-7721 cells was markedly suppressed by oligochitosan.In experiment in vivo,oligochitosan inhibited the growth of H22 tumor cells,the tumor inhibiting rate of high doses of oligochitosan(300mg/kg) was 33.13% and the expression of Bcl-2 was markedly suppressed in the tumor tissues by oligochitosan.Conclusion Oligochitosan both in vivo and in vitro inhibits the proliferation and induces the apoptosis of hepatocellular carcinoma cells.The mechanism is possibly that oligochitosan down-regulates the expression of Bcl-2.
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