Effects of Five Kinds of Traditional Chinese Medicine on Proliferation Activity in Vitro of Bone Marrow Mesenchymal Stem Cells in Rats

Objective:To investigate the effects of Radix Astragali,Salvia,Herba Epimedii,compound Youguiyin serum and its decoction,Xuelianhua Decoction and its ethanolic extract on proliferative activity in vitro of bone marrow mesenchymal stem cells in rat.Methods:Through high pressure sterilization,after Saussurea Involucrate soaked in ethanol and other single herb and compound decoction were administered to SD rats by 10 times of the routine dose to do the preparation of herb serum.The bone marrow mesenchymal stem cells(MSCs) were isolated and cultured from rat femur and humerus by conventional method.The herb serum administered to the P3 MSCs with 15%、10%、5% and 1% concentration group.The ethanolic extract of Saussurea Involucrate and other single herb and compound decoction were administered to the P3 MSCs with10-2、10-3、10-4、10-5 concentration group.The cells proliferative activity were determined by MTT method after having been cultured 3 days.Results:The herb serum and the ethanolic extract of Saussurea Involucrate promoted MSCs cell proliferation with all concentration groups.Conclusion:Saussurea Involucrate showed the positive effect on rat bone marrow mesenchymal stem cells proliferative activity in vitro.