Triplex PCR Detection of Ralstonia solanacearum,Phytophthora nicotianae and Pythium aphanidermatum from Infected Tobacco Tissues

R. solanacearum,P. nicotianae and P. aphanidermatum are important compound infection diseases of the tobacco production. In this study,Three pairs of primers were designed respectively according to the genomic DNA of R. solanacearum,P. nicotiana and P. aphanidermatum. And they were used to amplify simultaneously the DNA sequences by multiplex PCR and the reaction conditions were optimized. The results showed that the multiplex PCR assay amplified three specific target channels of 461,364 and 265 bp. The detection limits of the multiplex PCR for R. solanacearum,P. nicotiana and P. aphanidermatum were 1. 01 ng / μL. This multiplex PCR method was accurate,fast and effective and provided a new approach and an important guiding role for the tobacco disease compound infection diagnosis and treatment.