假体周围
抗生素
微生物学
关节感染
酶
药品
医学
材料科学
外科
化学
药理学
生物
关节置换术
生物化学
作者
Elif Sarıgöl,Melike Ekizoğlu,Sibel Bozdağ Pehlivan,Ebru Bodur,Meral Sağıroğlu,Sema Çalış
标识
DOI:10.1016/j.jddst.2017.11.004
摘要
Biofilms have an important role in the pathogenesis of Periprosthetic Joint Infection (PJI)s and are very likely the main cause in resistance to conventional treatments. In this study, a novel thermosensitive, Pluronic - based gel formulation, containing an enzyme (Serratiopeptidase, SP), an antibiotic drug (Vancomycin HCl, VA) and antibiotic-loaded microspheres (TG-EAM) were prepared and evaluated for their effect against PJI. Viscosity measurements, drug and enzyme release, in vitro enzyme activity and in vitro anti-biofilm activity studies were performed to characterize the formulations. While in vitro drug release studies showed that 74.5% VA was released after 25 d from TG-EAM, the in vitro enzyme activity was measurable for two days. Based on the obtained experimental results, the formulation containing 0.8% (w/v) free VA, 5% (w/v) SP and 40% (w/v) of VA loaded microspheres was chosen as the most suitable formulation for future in vivo studies, as a promising alternative to current standard treatments for PJI.
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