生物
胎盘碱性磷酸酶
报告基因
碱性磷酸酶
转染
基因
基因表达
分子生物学
磷酸酶
生物搬运器
信使核糖核酸
细胞生物学
酶
生物化学
作者
J Berger,Joachim Hauber,Regina Hauber,R. Geiger,Bryan R. Cullen
出处
期刊:Gene
[Elsevier]
日期:1988-06-01
卷期号:66 (1): 1-10
被引量:686
标识
DOI:10.1016/0378-1119(88)90219-3
摘要
This paper describes a novel eukaryotic reporter gene, secreted alkaline phosphatase (SEAP). In transient expression experiments using transfected mammalian cells, we demonstrate that SEAP yields results that are qualitatively and quantitatively similar, at both the mRNA and protein levels, to parallel results obtained using established reporter genes. However, SEAP offers significant advantages in terms of ease of assay and assay expense, and also has the potential for quantitative assay at levels as low as 0.2 pg/ml of culture medium. These attributes suggest that SEAP may have general utility in experiments which rely on the accurate measurement of reporter gene expression levels.
科研通智能强力驱动
Strongly Powered by AbleSci AI