生物
热启动PCR
底漆二聚体
硅胶PCR
多重位移放大
寡核苷酸
PCR的应用
分子生物学
聚合酶链反应
底漆(化妆品)
DNA
基因组
遗传学
多重聚合酶链反应
基因
DNA提取
化学
有机化学
作者
H. Telenius,Nigel P. Carter,Charlotte E. Bebb,Magnus Nordenskjo ̈ld,Bruce A.J. Ponder,Alan Tunnacliffe
出处
期刊:Genomics
[Elsevier]
日期:1992-07-01
卷期号:13 (3): 718-725
被引量:1337
标识
DOI:10.1016/0888-7543(92)90147-k
摘要
A version of the polymerase chain reaction (PCR), termed degenerate oligonucleotide-primed PCR (DOP-PCR), which employs oligonucleotides of partially degenerate sequence, has been developed for genome mapping studies. This degeneracy, together with a PCR protocol utilizing a low initial annealing temperature, ensures priming from multiple (e.g., ∼106 in human) evenly dispersed sites within a given genome. Furthermore, as efficient amplification is achieved from the genomes of all species tested using the same primer, the method appears to be species-independent. Thus, for the general amplification of target DNA, DOP-PCR has advantages over interspersed repetitive sequence PCR (IRS-PCR), which relies on the appropriate positioning of species-specific repeat elements. In conjunction with chromosome flow sorting, DOP-PCR has been applied to the characterization of abnormal chromosomes and also to the cloning of new markers for specific chromosome regions. DOP-PCR therefore represents a rapid, efficient, and species-independent technique for general DNA amplification.
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